Research

The Klein Omics Lab’s current research deals with a range of topics, most namely the detection and identification of food microbes, studies on metabolic effects of functional foods, metabolic aspects of gut microbiota dysbiosis, metabolomics analyses on maternal health, and metabolism studies of mosquito diapause.

 

Journal Articles

Shearer, J.; Klein, M.S.; Vogel, H.J.; Mohammad, S.; Bainbridge, S.; Adamo, K.B., “Maternal and Cord Blood Metabolites as Predictors of Gestational Weight Gain and Pregnancy Health Outcomes: A Pilot Study” Journal of Proteome Research (2021), https://doi.org/10.1021/acs.jproteome.0c00854 

 

Wang, D.; Greenwood, P.; Klein, M.S., “A Protein-free Chemically Defined Medium for the Cultivation of Various Microorganisms with Food Safety Significance” Journal of Applied Microbiology (2021), https://doi.org/10.1111/jam.15005

 
Huck, D.T.; Klein, M.S.; Meuti, M.E., “Determining the Effects of Nutrition on the Reproductive Physiology of Male Mosquitoes” Journal of Insect Physiology 129 (2021) 104191, 

 

Klein, M.S., “Affine Transformation of Negative Values for NMR Metabolomics Using the mrbin R Package” Journal of Proteome Research 20 (2021) 1397-1404, https://doi.org/10.1021/acs.jproteome.0c00684

 

Shearer, J.; Reimer, R.A.; Hittel, D.S.; Gault, M.A.; Vogel, H.J.; Klein M.S., “Caffeine-Containing Energy Shots Cause Acute Impaired Glucoregulation in Adolescents” Nutrients 12 (2020) 3850, https://doi.org/10.3390/nu12123850

 

Lee, A.H.; Dhingra, S.K.; Lewis, I.A.; Singh, M.K.; Siriwardana, A.; Dalal, S.; Rubiano, K.; Klein, M.S.; Baska, K.S.; Krishna, S.; Klemba, M. et al., “Evidence for Regulation of Hemoglobin Metabolism and Intracellular Ionic Flux by the Plasmodium falciparum Chloroquine Resistance Transporter” Scientific Reports (2018) 13578, https://doi.org/10.1038/s41598-018-31715-9

 

Newell, C.; Sabouny, R.; Hittel, D.S.; Shutt, T.E.; Khan, A.; Klein, M.S.; Shearer, J., “Mesenchymal stem cells shift mitochondrial dynamics and enhance oxidative phosphorylation in recipient cells” Frontiers in Physiology 9 (2018) 1572, https://doi.org/10.3389/fphys.2018.01572

 

Schober, D.; Jacob, D.; Wilson, M.; Cruz, J.A.; Marcu, A.; Grant, J.R.; Moing, A.; Deborde, C.; de Figueiredo, L.F.; Haug K.; Rocca-Serra, P.; Easton, J.; Ebbels, T.M.D.; Hao, J.; Ludwig, C. et al., “nmrML: A Community Supported Open Data Standard for the Description, Storage, and Exchange of NMR Data.” Anal Chem 90 (2018) 649-656, https://doi.org/10.1021/acs.analchem.7b02795

 

Newell, C.; Johnsen, V.L.; Yee, N.C.; Xu, W.J.; Klein, M.S.; Khan, A.; Rho, J.M.; Shearer, J., “Ketogenic diet leads to O-GlcNAc modification in the BTBRT+tf/j mouse model of autism.” Biochim Biophys Acta Mol Basis Dis 1863 (2017) 2274-2281, https://doi.org/10.1016/j.bbadis.2017.05.013

 

Lu, W.; Su, X.; Klein, M.S.; Lewis, I.A. et al., “Metabolite measurement: Pitfalls to avoid and practices to follow” Annual Review of Biochemistry 86 (2017) 277-304, https://doi.org/10.1146/annurev-biochem-061516-044952

 

Beatty, P.H.; Klein, M.S.; Fischer, J.J.; Lewis, I.A. et al., “Understanding Plant Nitrogen Metabolism through Metabolomics and Computational Approaches.” Plants (Basel) (2016), https://doi.org/10.3390/plants5040039

 

Klein, M.S.; Shearer, J., “Metabolomics and Type 2 Diabetes: Translating Basic Research into Clinical Application.” J Diabetes Res 2016 (2016) 3898502, https://doi.org/10.1155/2016/3898502

 

Klein, M.S.; Newell, C.; Bomhof, M.R.; Reimer, R.A.; Hittel, D.S.; Rho, J.M.; Vogel, H.J.; Shearer, J., “Metabolomic Modelling to Monitor Host Responsiveness to Gut Microbiota Manipulation in the BTBR T+tf /j Mouse” Journal of Proteome Research 15 (2016) 1143-1150, https://doi.org/10.1021/acs.jproteome.5b01025

 

Tetens, J.; Heuer, C.; Heyer, I.; Klein, M.S.; Gronwald, W.; Junge, W.; Oefner, P.J.; Thaller, G.; Krattenmacher, N., “Polymorphisms within the APOBR gene are highly associated with milk levels of prognostic ketosis biomarkers in dairy cows.” Physiol Genomics 47 (2015) 129-137, https://doi.org/10.1152/physiolgenomics.00126.2014

 

Ehret, A.; Hochstuhl, D.; Krattenmacher, N.; Tetens, J. et al., “Use of genomic and metabolic information as well as milk performance records for prediction of subclinical ketosis risk via artificial neural networks.” J Dairy Sci 98 (2015) 322-329, https://doi.org/10.3168/jds.2014-8602

 

Deschamps, C.L.; Connors, K.E.; Klein, M.S.; Johnsen, V.L.; Shearer, J.; Vogel, H.J.; Devaney, J.M.; Gordish-Dressman, H.; Many, G.M.; Barfield, W. et al., “The ACTN3 R577X Polymorphism Is Associated with Cardiometabolic Fitness in Healthy Young Adults.” PLoS One 10 (2015) e0130644, https://doi.org/10.1371/journal.pone.0130644

 

Klein, M.S.; Connors, K.E.; Shearer, J.; Vogel, H.J. et al., “Metabolomics reveals the sex-specific effects of the SORT1 low-density lipoprotein cholesterol locus in healthy young adults.” J Proteome Res 13 11 (2014) 5063-5070, https://doi.org/10.1021/pr500659r

 

Klein, M.S.; Almstetter, M.F.; Nürnberger, N.; Sigl, G.; Gronwald, W.; Wiedemann, S.; Dettmer, K.; Oefner, P.J., “Correlations between milk and plasma levels of amino and carboxylic acids in dairy cows.” J Proteome Res 12 11 (2013) 5223-5232, https://doi.org/10.1021/pr4006537

 

Zacharias, H.U.; Schley, G.; Hochrein, J.; Klein, M.S.; Köberle, C.; Eckardt, K.U.; Willam, C.; Oefner, P.J.; Gronwald, W., “Analysis of human urine reveals metabolic changes related to the development of acute kidney injury following cardiac surgery” Metabolomics (2013) 697-707, https://doi.org/10.1007/s11306-012-0479-4

 

Klein, M.S.; Oefner, P.J.; Gronwald, W., “MetaboQuant: a tool combining individual peak calibration and outlier detection for accurate metabolite quantification in 1D (1)H and (1)H-(13)C HSQC NMR spectra.” Biotechniques 54 (2013) 251-256, https://doi.org/10.2144/000114026

 

Thomas, A.; Klein, M.S.; Stevens, A.P.; Reinders, Y.; Hellerbrand, C.; Dett, K.; Gronwald, W.; Oefner, P.J.; Reinders, J., “Changes in the hepatic mitochondrial and membrane proteome in mice fed a non-alcoholic steatohepatitis inducing diet.” J Proteomics 80 (2013) 107-122, https://doi.org/10.1016/j.jprot.2012.12.027

 

Hochrein, J.; Klein, M.S.; Zacharias, H.U.; Li, J.; Wijffels, G.; Schirra, H. R.; Oefner, P.J.; Gronwald, W., “Performance evaluation of algorithms for the classification of metabolic 1H NMR fingerprints.” J Proteome Res 11 12 (2012) 6242-6251, https://doi.org/10.1021/pr3009034

 

Kohl, S.M.; Klein, M.S.; Hochrein, J.; Oefner, P.J. et al., “State-of-the art data normalization methods improve NMR-based metabolomic analysis.” Metabolomics Suppl 1 (2012) 146-160, https://doi.org/10.1007/s11306-011-0350-z

 

Thomas, A.; Stevens, A.P.; Klein, M.S.; Hellerbrand, C.; Dettmer, K.; Gronwald, W.; Oefner, P.J.; Reinders, J., “Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet.” Proteomics 12 (2012) 1437-1451, https://doi.org/10.1002/pmic.201100628

 

Klein, M.S.; Buttchereit, N.; Miemczyk, S.P.; Immervoll, A-K.; Louis, C.; Wiedemann, S.; Junge, W.; Thaller, G.; Oefner, P.J.; Gronwald, W., “NMR metabolomic analysis of dairy cows reveals milk glycerophosphocholine to phosphocholine ratio as prognostic biomarker for risk of ketosis.” J Proteome Res 11 (2012) 1373-1381, https://doi.org/10.1021/pr201017n

 

Klein, M.S.; Dorn, C.; Saugspier, M.; Hellerbrand, C. et al., “Discrimination of steatosis and NASH in mice using nuclear magnetic resonance spectroscopy” Metabolomics (2011) 237-246, https://doi.org/10.1007/s11306-010-0243-6

 

Gronwald, W.; Klein, M.S.; Zeltner, R.; Schulze, B-D.; Reinhold, S.W.; Deutschmann, M.; Immervoll, A.-K.; Böger, C.A. et al., “Detection of autosomal dominant polycystic kidney disease by NMR spectroscopic fingerprinting of urine.” Kidney Int 79 (2011) 1244-1253, https://doi.org/10.1038/ki.2011.30

 

Klein, M.S.; Almstetter, M.F.; Schlamberger, G.; Nürnberger, N.; Dettmer, K.; Oefner, P.J.; Meyer, H.H.D.; Wiedeman, S.; Gronwald, W., “Nuclear magnetic resonance and mass spectrometry-based milk metabolomics in dairy cows during early and late lactation.” J Dairy Sci 93 (2010) 1539-1550, https://doi.org/10.3168/jds.2009-2563

 

Gronwald, W.; Klein, M.S.; Kaspar, H.; Fagerer, S.R.; Nürnberger, N.; Dettmer, K.; Bertsch, T.; Oefner, P.J., “Urinary metabolite quantification employing 2D NMR spectroscopy.” Anal Chem 80 23 (2008) 9288-9297, https://doi.org/10.1021/ac801627c