Research

The Klein Omics Lab’s current research deals with a range of topics, most namely the detection and identification of food microbes, studies on metabolic effects of functional foods, metabolic aspects of gut microbiota dysbiosis, metabolomics analyses on maternal health, and metabolism studies of mosquito diapause.

 

Journal Articles

Lee, A.H.; Dhingra, S.K.; Lewis, I.A.; Singh, M.K.; Siriwardana, A.; Dalal, S.; Rubiano, K.; Klein, M.S.; Baska, K.S.; Krishna, S.; Klemba, M. et al., “Evidence for Regulation of Hemoglobin Metabolism and Intracellular Ionic Flux by the Plasmodium falciparum Chloroquine Resistance Transporter” Scientific Reports (2018) 13578
 
Newell, C.; Sabouny, R.; Hittel, D.S.; Shutt, T.E.; Khan, A.; Klein, M.S.; Shearer, J., “Mesenchymal stem cells shift mitochondrial dynamics and enhance oxidative phosphorylation in recipient cells” Frontiers in Physiology NOV (2018) 1572
 
Schober, D.; Jacob, D.; Wilson, M.; Cruz, J.A.; Marcu, A.; Grant, J.R.; Moing, A.; Deborde, C.; de Figueiredo, L.F.; Haug K.; Rocca-Serra, P.; Easton, J.; Ebbels, T.M.D.; Hao, J.; Ludwig, C. et al., “nmrML: A Community Supported Open Data Standard for the Description, Storage, and Exchange of NMR Data.” Anal Chem 90 (2018) 649-656
 
Newell, C.; Johnsen, V.L.; Yee, N.C.; Xu, W.J.; Klein, M.S.; Khan, A.; Rho, J.M.; Shearer, J., “Ketogenic diet leads to O-GlcNAc modification in the BTBRT+tf/j mouse model of autism.” Biochim Biophys Acta Mol Basis Dis 1863 (2017) 2274-2281
 
Lu, W.; Su, X.; Klein, M.S.; Lewis, I.A. et al., “Metabolite measurement: Pitfalls to avoid and practices to follow” Annual Review of Biochemistry 86 (2017) 277-304
 
Beatty, P.H.; Klein, M.S.; Fischer, J.J.; Lewis, I.A. et al., “Understanding Plant Nitrogen Metabolism through Metabolomics and Computational Approaches.” Plants (Basel) (2016)
 
Klein, M.S.; Shearer, J., “Metabolomics and Type 2 Diabetes: Translating Basic Research into Clinical Application.” J Diabetes Res 2016 (2016) 3898502
 
Klein, M.S.; Newell, C.; Bomhof, M.R.; Reimer, R.A.; Hittel, D.S.; Rho, J.M.; Vogel, H.J.; Shearer, J., “Metabolomic Modelling to Monitor Host Responsiveness to Gut Microbiota Manipulation in the BTBR T+tf /j Mouse” JOURNAL OF PROTEOME RESEARCH 15 (2016) 1143-1150
 
Tetens, J.; Heuer, C.; Heyer, I.; Klein, M.S.; Gronwald, W.; Junge, W.; Oefner, P.J.; Thaller, G.; Krattenmacher, N., “Polymorphisms within the APOBR gene are highly associated with milk levels of prognostic ketosis biomarkers in dairy cows.” Physiol Genomics 47 (2015) 129-137
 
Ehret, A.; Hochstuhl, D.; Krattenmacher, N.; Tetens, J. et al., “Short communication: Use of genomic and metabolic information as well as milk performance records for prediction of subclinical ketosis risk via artificial neural networks.” J Dairy Sci 98 (2015) 322-329
 
Deschamps, C.L.; Connors, K.E.; Klein, M.S.; Johnsen, V.L.; Shearer, J.; Vogel, H.J.; Devaney, J.M.; Gordish-Dressman, H.; Many, G.M.; Barfield, W. et al., “The ACTN3 R577X Polymorphism Is Associated with Cardiometabolic Fitness in Healthy Young Adults.” PLoS One 10 (2015) e0130644
 
Klein, M.S.; Connors, K.E.; Shearer, J.; Vogel, H.J. et al., “Metabolomics reveals the sex-specific effects of the SORT1 low-density lipoprotein cholesterol locus in healthy young adults.” J Proteome Res 13 11 (2014) 5063-5070
 
Klein, M.S.; Almstetter, M.F.; Nürnberger, N.; Sigl, G.; Gronwald, W.; Wiedemann, S.; Dettmer, K.; Oefner, P.J., “Correlations between milk and plasma levels of amino and carboxylic acids in dairy cows.” J Proteome Res 12 11 (2013) 5223-5232
 
Zacharias, H.U.; Schley, G.; Hochrein, J.; Klein, M.S.; Köberle, C.; Eckardt, K.U.; Willam, C.; Oefner, P.J.; Gronwald, W., “Analysis of human urine reveals metabolic changes related to the development of acute kidney injury following cardiac surgery” Metabolomics (2013) 697-707
 
Klein, M.S.; Oefner, P.J.; Gronwald, W., “MetaboQuant: a tool combining individual peak calibration and outlier detection for accurate metabolite quantification in 1D (1)H and (1)H-(13)C HSQC NMR spectra.” Biotechniques 54 (2013) 251-256
 
Thomas, A.; Klein, M.S.; Stevens, A.P.; Reinders, Y.; Hellerbrand, C.; Dett, K.; Gronwald, W.; Oefner, P.J.; Reinders, J., “Changes in the hepatic mitochondrial and membrane proteome in mice fed a non-alcoholic steatohepatitis inducing diet.” J Proteomics 80 (2013) 107-122
 
Hochrein, J.; Klein, M.S.; Zacharias, H.U.; Li, J.; Wijffels, G.; Schirra, H. R.; Oefner, P.J.; Gronwald, W., “Performance evaluation of algorithms for the classification of metabolic 1H NMR fingerprints.” J Proteome Res 11 12 (2012) 6242-6251
 
Kohl, S.M.; Klein, M.S.; Hochrein, J.; Oefner, P.J. et al., “State-of-the art data normalization methods improve NMR-based metabolomic analysis.” Metabolomics Suppl 1 (2012) 146-160
 
Thomas, A.; Stevens, A.P.; Klein, M.S.; Hellerbrand, C.; Dettmer, K.; Gronwald, W.; Oefner, P.J.; Reinders, J., “Early changes in the liver-soluble proteome from mice fed a nonalcoholic steatohepatitis inducing diet.” Proteomics 12 (2012) 1437-1451
 
Klein, M.S.; Buttchereit, N.; Miemczyk, S.P.; Immervoll, A-K.; Louis, C.; Wiedemann, S.; Junge, W.; Thaller, G.; Oefner, P.J.; Gronwald, W., “NMR metabolomic analysis of dairy cows reveals milk glycerophosphocholine to phosphocholine ratio as prognostic biomarker for risk of ketosis.” J Proteome Res 11 (2012) 1373-1381
 
Klein, M.S.; Dorn, C.; Saugspier, M.; Hellerbrand, C. et al., “Discrimination of steatosis and NASH in mice using nuclear magnetic resonance spectroscopy” Metabolomics (2011) 237-246
 
Gronwald, W.; Klein, M.S.; Zeltner, R.; Schulze, B-D.; Reinhold, S.W.; Deutschmann, M.; Immervoll, A.-K.; Böger, C.A. et al., “Detection of autosomal dominant polycystic kidney disease by NMR spectroscopic fingerprinting of urine.” Kidney Int 79 11 (2011) 1244-1253-1244-1253
Klein, M.S.; Almstetter, M.F.; Schlamberger, G.; Nürnberger, N.; Dettmer, K.; Oefner, P.J.; Meyer, H.H.D.; Wiedeman, S.; Gronwald, W., “Nuclear magnetic resonance and mass spectrometry-based milk metabolomics in dairy cows during early and late lactation.” J Dairy Sci 93 (2010) 1539-1550
 
Gronwald, W.; Klein, M.S.; Kaspar, H.; Fagerer, S.R.; Nürnberger, N.; Dettmer, K.; Bertsch, T.; Oefner, P.J., “Urinary metabolite quantification employing 2D NMR spectroscopy.” Anal Chem 80 23 (2008) 9288-9297